E-RNAi: a web application to design optimized RNAi constructs
نویسندگان
چکیده
منابع مشابه
E-RNAi: a web application to design optimized RNAi constructs
RNA interference (RNAi) has become a powerful genetic approach to systematically dissect gene function on a genome-wide scale. Owing to the penetrance and efficiency of RNAi in invertebrates, model organisms such as Drosophila melanogaster and Caenorhabditis elegans have contributed significantly to the identification of novel components of diverse biological pathways, ranging from early develo...
متن کاملE-RNAi: a web application for the multi-species design of RNAi reagents—2010 update
The design of RNA interference (RNAi) reagents is an essential step for performing loss-of-function studies in many experimental systems. The availability of sequenced and annotated genomes greatly facilitates RNAi experiments in an increasing number of organisms that were previously not genetically tractable. The E-RNAi web-service, accessible at http://www.e-rnai.org/, provides a computationa...
متن کامل[Application of RNAi library to oncology investigation].
RNA interference (RNAi) library was pioneered in C. elegans in a broad range of organism-based screens. During the past few years, RNAi has become a powerful tool to silence the expression of genes and analyze their loss-of-function phenotype in mammalian cells. There are two types of RNAi library, synthetic oligonucleotide library and vector library, and two screening strategies, high throughp...
متن کاملAsian Citrus Psyllid RNAi Pathway – RNAi evidence
Diaphorina citri, known as the Asian citrus psyllid, is an important pest of citrus because it transmits a phloem-limited bacteria strongly implicated in huanglongbing (citrus greening disease). Emerging biotechnologies, such as RNA interference, could provide a new sustainable and environmentally friendly strategy for the management of this pest. In this study, genome and functional analysis w...
متن کاملBacterial Delivery of RNAi Effectors: Transkingdom RNAi
RNA interference (RNAi) represents a high effective mechanism for specific inhibition of mRNA expression. Besides its potential as a powerful laboratory tool, the RNAi pathway appears to be promising for therapeutic utilization. For development of RNA interference (RNAi)-based therapies, delivery of RNAi-mediating agents to target cells is one of the major obstacles. A novel strategy to overcom...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Nucleic Acids Research
سال: 2005
ISSN: 0305-1048,1362-4962
DOI: 10.1093/nar/gki468